| Environmental DNA swab | Tropilaelaps mites | Is it there? | Swabs of comb surfaces, sent to a laboratory for DNA testing. | 100%. One field trial: 27 infested colonies in six apiaries, Eastern Highlands of Papua New Guinea, May 2025, mean worker-brood infestation 6 ± 3%. Detection at low infestation is harder. | Sample sent to a laboratory | Laboratory | The most sensitive method in the trial, and the only one to find the mite at low infestation, but it needs a laboratory and cost about A$28.75 per test. |
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| Sticky mat after an authorised miticide | Tropilaelaps mites | Is it there? | A sticky mat on the hive floor, collected after 24 hours. | 92%. One field trial: 27 infested colonies in six apiaries, Eastern Highlands of Papua New Guinea, May 2025, mean worker-brood infestation 6 ± 3%. Detection at low infestation is harder. | Non-destructive | Practice needed | The most expensive method in the trial (about A$57.08 per test). The authors warn that resistance to miticides could weaken detection that relies on them. |
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| Worker brood uncapping | Tropilaelaps mites | Is it there? | Sealed worker brood uncapped cell by cell. | 70% (about 86% if 200 cells are uncapped). One field trial: 27 infested colonies in six apiaries, Eastern Highlands of Papua New Guinea, May 2025, mean worker-brood infestation 6 ± 3%. Detection at low infestation is harder. Another trial: 92.3% at 200 cells; 85% at 100 cells (A second trial: 26 infested colonies at Chiang Mai University, Thailand, over ten days in January 2024, with 200-cell uncapping as the reference.). | Destroys the sampled brood | Practice needed | Sensitivity depends on how many cells are opened. |
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| Rapid brood uncapping | Tropilaelaps mites | Is it there? | A strip of sealed brood uncapped in one pass. | 63%. One field trial: 27 infested colonies in six apiaries, Eastern Highlands of Papua New Guinea, May 2025, mean worker-brood infestation 6 ± 3%. Detection at low infestation is harder. | Destroys the sampled brood | Practice needed | The cheapest method in the trial (about A$6.87 per test), but it needed about four repeat tests for 95% confidence. |
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| Alcohol wash | Tropilaelaps mites | Is it there? | A sample of adult bees, washed as for varroa. | 41%. One field trial: 27 infested colonies in six apiaries, Eastern Highlands of Papua New Guinea, May 2025, mean worker-brood infestation 6 ± 3%. Detection at low infestation is harder. Another trial: 38.5% (A second trial: 26 infested colonies at Chiang Mai University, Thailand, over ten days in January 2024, with 200-cell uncapping as the reference.). | Kills the sampled bees | Any beekeeper | Aimed at the wrong place: Tropilaelaps lives on brood, not adults. About six washes were needed for 95% confidence. |
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| Bump test | Tropilaelaps mites | Is it there? | A brood frame knocked sharply over a tray, and the tray searched for mites. | 33%. One field trial: 27 infested colonies in six apiaries, Eastern Highlands of Papua New Guinea, May 2025, mean worker-brood infestation 6 ± 3%. Detection at low infestation is harder. Another trial: 73.1% (A second trial: 26 infested colonies at Chiang Mai University, Thailand, over ten days in January 2024, with 200-cell uncapping as the reference.). | Non-destructive | Any beekeeper | The least sensitive method in the Papua New Guinea trial, needing about eight tests for 95% confidence — yet it found the mite in nearly three-quarters of colonies in the Thai trial. The two trials differ in place, season, infestation and how the tray was searched, and neither explains the gap. |
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| Sticky board under the colony, without a miticide | Tropilaelaps mites | Is it there? | A greased board under a mesh floor, left for about three days and searched for mites. | 100%. A second trial: 26 infested colonies at Chiang Mai University, Thailand, over ten days in January 2024, with 200-cell uncapping as the reference. | Non-destructive | Practice needed | The most sensitive method in the Thai trial, but slow to search, and the mite is about a third of varroa's size. Not the same method as the mat after a miticide in the other trial. |
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| Powdered sugar shake | Tropilaelaps mites | Is it there? | About 300 adult bees shaken in powdered sugar; the bees are returned. | 34.6%. A second trial: 26 infested colonies at Chiang Mai University, Thailand, over ten days in January 2024, with 200-cell uncapping as the reference. | Non-destructive | Any beekeeper | Samples adult bees, where Tropilaelaps spends little time. The same bees found varroa in 96% of colonies, which is why a method that works for varroa says little about this mite. |
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| Sentinel apiaries | Small hive beetle; Tropilaelaps mites | Is it there? | Colonies kept near likely points of entry, such as ports, and inspected regularly by the authority. | Not quantified. | Non-destructive | Practice needed | Covers places, not the whole territory. 'Not detected' from sentinel apiaries is a surveillance result, not proof of absence. |
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