- Pollen of the named plant
- Share of the pollen that comes from the plant the honey is named after. Varies enormously by plant — chestnut over-represents its pollen, citrus and lavender under-represent theirs — which is why no single percentage defines 'monofloral'.
- Colour
- Depth of colour on the Pfund scale — 0 is water-white, above 114 is dark amber. Tracks minerals and phenolic compounds, and darkens with heat and age.
- Electrical conductivity
- How well a honey solution carries current, which rises with its mineral and acid content. The main official test separating honeydew and chestnut honeys (high) from most blossom honeys (low).
- Specific rotation
- The direction in which the sugars turn polarised light. Fructose turns it left, so blossom honeys read negative; honeydew honeys carry oligosaccharides that turn it right and read positive.
- pH
- Acidity as a logarithmic scale. Honey is always acidic; honeydew and chestnut honeys sit highest because their minerals buffer the acids.
- Free acidity
- The acid present as free acid, mostly gluconic acid made by the bee enzyme glucose oxidase. Rises with fermentation, which is why it has a legal ceiling.
- Lactones
- Acid held in lactone form — the reserve that free-acidity titration does not see. Added to free acidity to give total acidity.
- Total acidity
- Free acidity plus lactones.
- Water
- Moisture content. Decides whether a honey can ferment and how fast it pours; set by the nectar, the weather and how long the bees ripened it.
- Diastase activity
- Activity of the starch-digesting enzyme the bees add. Falls with heating and storage, so a low value in a honey that should be high suggests heat or age — but some honeys, citrus and acacia among them, are naturally low.
- Invertase activity
- Activity of the bee enzyme that splits sucrose into glucose and fructose. More heat-sensitive than diastase, and a sharper indicator of freshness and gentle handling.
- Proline
- The main free amino acid, mostly from the bees' own secretions. A low value is one signal of sugar-syrup dilution or of an unripe honey.
- Fructose
- The sweeter, more soluble of the two main sugars. Honeys rich in it stay liquid.
- Glucose
- The less soluble of the two main sugars and the one that crystallises. Honeys rich in it set quickly and finely.
- Sucrose
- Ordinary sugar, mostly split by the bees during ripening. A few honeys — lavender, acacia, citrus — keep more of it naturally, which is why their legal limits are higher.
- Fructose + glucose
- The two main sugars together. Lower in honeydew honeys, which carry more oligosaccharides — melezitose, erlose and others — made by the insects that produced the honeydew.
- Fructose ÷ glucose
- The single best predictor of whether a honey crystallises. Below about 1.1 it sets quickly; above about 1.3 it tends to stay liquid for a long time.
- Glucose ÷ water
- How much glucose there is for each part of water. Above about 2.1 the solution is supersaturated enough to crystallise fast; below about 1.7 it crystallises slowly or not at all.